Demonstration of seed inoculation of dimer infectious clones of mungbean yellow mosaic India virus-soybean isolate.

ABSTRACT: In this study, the full-length components of mungbean yellow mosaic India virus (MYMIV) DNA-A (MW590720 & MW600934) and DNA-B (MW659819 & MW659820) from a soybean isolate were cloned and sequenced. Nucleotide sequence analysis of both MYMIV components revealed > 96% identity and close ancestry with MYMIV isolates from legumes in Southeast Asia. Furthermore, dimeric infectious clones of MYMIV were generated in the pCAMBIA1302 vector, and a seed infiltration protocol was established for mungbean, soybean, and Nicotiana tabacum. Agroinfiltration induced yellow mosaic symptoms in mungbean and N. tabacum plants 3 weeks post-infiltration, which were further confirmed by PCR using MYMIV-specific DNA primers.

Supplementary information

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SUBMITTER: Gupta T 

PROVIDER: S-EPMC10567615 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature