Infiltrating macrophages amplify doxorubicin-induced cardiac damage: role of catecholamines.

ABSTRACT:

Background

The functional contribution of non-myocyte cardiac cells, such as inflammatory cells, in the setup of heart failure in response to doxorubicin (Dox) is recently becoming of growing interest.

Objectives

The study aims to evaluate the role of macrophages in cardiac damage elicited by Dox treatment.

Methods

C57BL/6 mice were treated with one intraperitoneal injection of Dox (20 mg/kg) and followed up for 5 days by cardiac ultrasounds (CUS), histological, and flow cytometry evaluations. We also tested the impact of Dox in macrophage-depleted mice. Rat cardiomyoblasts were directly treated with Dox (D-Dox) or with a conditioned medium from cultured murine macrophages treated with Dox (M-Dox).

Results

In response to Dox, macrophage infiltration preceded ca

SUBMITTER: Gambardella J 

PROVIDER: S-EPMC10567889 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature