Comparative metabolism of aflatoxin B₁ in mouse, rat and human primary hepatocytes using HPLC-MS/MS.

ABSTRACT: Aflatoxin B₁ (AFB₁) is a highly hepatotoxic and carcinogenic mycotoxin produced by Aspergillus species. The compound is mainly metabolized in the liver and its metabolism varies between species. The present study quantified relevant AFB₁- metabolites formed by mouse, rat, and human primary hepatocytes after treatment with 1 µM and 10 µM AFB₁. The use of liquid chromatographic separation coupled with tandem mass spectrometric detection enabled the selective and sensitive determination of phase I and phase II metabolites of AFB₁ over incubation times of up to 24 h. The binding of AFB₁ to macromolecules was also considered. The fastest metabolism of AFB₁ was observed in mouse hepatocytes which formed aflatoxin P

SUBMITTER: Gerdemann A 

PROVIDER: S-EPMC10567917 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature