Lung inflammation and interstitial fibrosis by targeted alveolar epithelial type I cell death.

ABSTRACT:

Introduction

The pathogenesis of chronic lung diseases is multifaceted with a major role of recurrent micro-injuries of the epithelium. While several reports clearly indicated a prominent role for surfactant-producing alveolar epithelial type 2 (AT2) cells, the contribution of gas exchange-permissive alveolar epithelial type 1 (AT1) cells has not been addressed yet. Here, we investigated whether repeated injury of AT1 cells leads to inflammation and interstitial fibrosis.

Methods

We chose an inducible model of AT1 cell depletion following local diphtheria toxin (DT) administration using an iDTR flox/flox (idTR^fl/fl) X Aquaporin 5CRE (Aqp5^CRE) transgenic mouse strain.

Results

We investigated repeated doses and intervals of DT to induce cell death

SUBMITTER: Carignon S 

PROVIDER: S-EPMC10568624 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature