A new method for the detection of Mycobacterium tuberculosis based on the CRISPR/Cas system.

ABSTRACT:

Object

Mycobacterium tuberculosis (MTB) is a bacterium that can cause zoonoses by aerosol transmission. Tuberculosis (TB) caused by MTB heavily burdens world public health security. Developing efficient, specific, convenient, and inexpensive MTB assays are essential for preventing and controlling TB.

Methods

In this study, we established a specific detection method for MTB using the Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) system, combined with recombinase mediated isothermal nucleic acid amplification (RAA) to improve the sensitivity of the detection system and achieve "two-level" amplification of the detection signal. The sensitivity and specificity of RAA combined with the CRISPR/Cas system were analyzed. Using BACTEC 960 culture as the gold sta

SUBMITTER: Zhang X 

PROVIDER: S-EPMC10568934 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature