A novel approach to enhance the performance of kallikrein 6 enzyme using Pichia pastoris GS115 as a host.

ABSTRACT:

Background and purpose

Enzyme engineering is the process of raising enzyme efficiency and activity by altering amino acid sequences. Kallikrein 6 (KLK6) enzyme is a secreted serine protease involved in a variety of physiological and pathological activities. The increased expression of KLK6 plays a key role in various diseases. Instability and spontaneous activation and deactivation are major challenges in the study of this enzyme. This study aimed to create a stable pro-KLK6 enzyme by enzyme engineering, designing a specific cleavage site for enterokinase, and using Pichia pastoris GS115 as a host cell. Then, recombinant pro-KLK6 was used to introduce a novel inhibitor for it.

Experimental approach

An engineered pro-KLK6 gene was cloned into the pPICZα A expression ve

SUBMITTER: Mahmoodi F 

PROVIDER: S-EPMC10568964 | biostudies-literature | 2023 Sep-Oct

REPOSITORIES: biostudies-literature