Aptamer Conformational Dynamics Modulate Neurotransmitter Sensing in Nanopores.

ABSTRACT: Aptamers that undergo conformational changes upon small-molecule recognition have been shown to gate the ionic flux through nanopores by rearranging the charge density within the aptamer-occluded orifice. However, mechanistic insight into such systems where biomolecular interactions are confined in nanoscale spaces is limited. To understand the fundamental mechanisms that facilitate the detection of small-molecule analytes inside structure-switching aptamer-modified nanopores, we correlated experimental observations to theoretical models. We developed a dopamine aptamer-functionalized nanopore sensor with femtomolar detection limits and compared the sensing behavior with that of a serotonin sensor fabricated with the same methodology. When these two neurotransmitters with comparable mass a

SUBMITTER: Stuber A 

PROVIDER: S-EPMC10569099 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature