Cytokines impact natural killer cell phenotype and functionality against glioblastoma in vitro.

ABSTRACT:

Objective

Natural killer (NK) cells are a part of the innate immune system and first-line defense against cancer. Since they possess natural mechanisms to recognize and kill tumor cells, NK cells are considered as a potential option for an off-the-shelf allogeneic cell-based immunotherapy. Here, our objective was to identify the optimal cytokine-based, feeder-free, activation and expansion protocol for cytotoxic NK cells against glioblastoma in vitro.

Methods

NK cells were enriched from human peripheral blood and expanded for 16 days with different activation and cytokine combinations. The expansion conditions were evaluated based on NK cell viability, functionality, expansion rate and purity. The cytotoxicity and degranulation of the expanded NK cells were measured <

SUBMITTER: Sivonen M 

PROVIDER: S-EPMC10569479 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature