Assessing variations in manual pipetting: An under-investigated requirement of good laboratory practice.

ABSTRACT: Pipettes are essential tools for biomedical and analytical laboratories, analogous to workstations for computer scientists. Variation in pipetting is a known unknown, as it is generally accepted that variations exist, but thus far, there have been limited studies on the extent of these variations in practice. In this mini-review, we highlight how manual pipetting is a key technique in the laboratory, and, although simple, inaccuracy and imprecision exist. If variations are not adequately addressed, errors can be compounded and consequently compromise data quality. Determination of the accuracy and precision of manual pipetting is straightforward, and here we review two common approaches that use gravimetry and spectrophotometry as readouts. We also provide detailed protocols for determinat

SUBMITTER: Guan XL 

PROVIDER: S-EPMC10569977 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature