TRNA queuosine modification is involved in biofilm formation and virulence in bacteria.

ABSTRACT: tRNA modifications are crucial for fine-tuning of protein translation. Queuosine (Q) modification of tRNAs is thought to modulate the translation rate of NAU codons, but its physiological role remains elusive. Therefore, we hypothesize that Q-tRNAs control those physiological processes involving NAU codon-enriched genes (Q-genes). Here, we report a novel bioinformatic strategy to predict Q-genes, revealing a widespread enrichment in functions, especially those related to biofilm formation and virulence in bacteria, and particularly in human pathogens. Indeed, we experimentally verified that these processes were significantly affected by altering the degree of tRNA Q-modification in different model bacteria, representing the first report of a general mechanism controlling biofilm formation

SUBMITTER: Diaz-Rullo J 

PROVIDER: S-EPMC10570037 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature