A novel splice variant of the human MSTN gene encodes a myostatin-specific myostatin inhibitor.

ABSTRACT:

Background

Myostatin, encoded by the MSTN gene comprising 3 exons, is a potent negative regulator of skeletal muscle growth. Although a variety of myostatin inhibitors have been invented for increasing muscle mass in muscle wasting diseases, no effective inhibitor is currently available for clinical use. Myostatin isoforms in several animals have been reported to inhibit myostatin, but an isoform has never been identified for the human MSTN gene, a conserved gene among animals. Here, a splice variant of the human MSTN gene was explored.

Methods

Transcripts and proteins were analysed by reverse transcription-PCR amplification and western blotting, respectively. Proteins were expressed from expression plasmid. Myostatin signalling was assayed by the SMAD-responsive luciferase

SUBMITTER: Maeta K 

PROVIDER: S-EPMC10570081 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature