AAV1.NT-3 gene therapy in the SOD1KO mouse model of accelerated sarcopenia.

ABSTRACT:

Background

Sarcopenia, an age-related loss of muscle mass, is a critical factor that affects the health of the older adults. The SOD1KO mouse is deficient of Cu/Zn superoxide dismutase, used as an accelerated aging model. We previously showed that NT-3 improves muscle fibre size by activating the mTOR pathway, suggesting a potential for attenuating age-related muscle loss. This study assessed the therapeutic efficacy of AAV1.NT-3 in this accelerated aging model.

Methods

Twelve 6 months old SOD1KO mice were injected intramuscularly with a 1 × 10¹¹ vg dose of AAV1.tMCK.NT-3, and 13 age-matched SOD1KO mice were used as controls. The treatment effect was evaluated using treadmill, rotarod and gait analyses as well as histological studies assessing changes in muscle fi

SUBMITTER: Tong L 

PROVIDER: S-EPMC10570084 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature