Site-Specific Photoaffinity Bioconjugation for the Creation of ⁸⁹Zr-Labeled Radioimmunoconjugates.

ABSTRACT:

Purpose

Site-specific approaches to bioconjugation produce well-defined and homogeneous immunoconjugates with potential for superior in vivo behavior compared to analogs synthesized using traditional, stochastic methods. The possibility of incorporating photoaffinity chemistry into a site-specific bioconjugation strategy is particularly enticing, as it could simplify and accelerate the preparation of homogeneous immunoconjugates for the clinic. In this investigation, we report the synthesis, in vitro characterization, and in vivo evaluation of a site-specifically modified, ⁸⁹Zr-labeled radioimmunoconjugate created via the reaction between an mAb and an Fc-binding protein bearing a photoactivatable 4-benzoylphenylalanine residue.

Procedures

A variant of the Fc-bind

SUBMITTER: Delaney S 

PROVIDER: S-EPMC10570397 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature