Purification of linearized template plasmid DNA decreases double-stranded RNA formation during IVT reaction.

ABSTRACT: After the COVID-19 pandemic, messenger RNA (mRNA) has revolutionized traditional vaccine manufacturing. With the increasing number of RNA-based therapeutics, valuable new scientific insights into these molecules have emerged. One fascinating area of study is the formation of double-stranded RNA (dsRNA) during in vitro transcription (IVT) which is considered a significant impurity, as it has been identified as a major trigger in the cellular immune response pathway. Therefore, there is a growing importance placed to develop and optimize purification processes for the removal of this by-product. Traditionally, efforts have primarily focused on mRNA purification after IVT through chromatographic separations, with anion exchange and reverse phase chromatography emerging as effective too

SUBMITTER: Martinez J 

PROVIDER: S-EPMC10570549 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature