Raman spectroscopy to assess the differentiation of bone marrow mesenchymal stem cells into a glial phenotype.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) are multipotent precursor cells with the ability to self-renew and differentiate into multiple cell linage, including the Schwann-like fate that promotes regeneration after lesion. Raman spectroscopy provides a precise characterization of the osteogenic, adipogenic, hepatogenic and myogenic differentiation of MSCs. However, the differentiation of bone marrow mesenchymal stem cells (BMSCs) towards a glial phenotype (Schwann-like cells) has not been characterized before using Raman spectroscopy.

Method

We evaluated three conditions: 1) cell culture from rat bone marrow undifferentiated (uBMSCs), and two conditions of differentiation; 2) cells exposed to olfactory ensheathing cells-conditioned medium (dBMSCs) and 3) cells obtained from o

SUBMITTER: Bautista-Gonzalez S 

PROVIDER: S-EPMC10570561 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature