Cell-permeable peptide nucleic acid antisense oligonucleotide platform targeting human betacoronaviruses.

ABSTRACT:

Introduction

Antisense oligonucleotides (ASOs) with therapeutic potential have recently been reported to target the SARS-CoV-2 genome. Peptide nucleic acids (PNAs)-based ASOs have been regarded as promising drug candidates, but intracellular delivery has been a significant obstacle. Here, we present novel modified PNAs, termed OPNAs, with excellent cell permeability that disrupt the RNA genome of SARS-CoV-2 and HCoV-OC43 by introducing cationic lipid moiety onto the nucleobase of PNA oligomer backbone.

Methods

HCT-8 cells and Caco-2 cells were treated with 1 μM antisense OPNAs at the time of viral challenge and the Viral RNA levels were measured by RT-qPCR three days post infection.

Results

NSP 14 targeting OPNA 5 and 11, reduced the viral titer to a half and OPNA 530

SUBMITTER: Park S 

PROVIDER: S-EPMC10570754 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature