"Influence of plasmids, selection markers and auxotrophic mutations on Haloferax volcanii cell shape plasticity".

ABSTRACT: Haloferax volcanii and other Haloarchaea can be pleomorphic, adopting different shapes, which vary with growth stages. Several studies have shown that H. volcanii cell shape is sensitive to various external factors including growth media and physical environment. In addition, several studies have noticed that the presence of a recombinant plasmid in the cells is also a factor impacting H. volcanii cell shape, notably by favoring the development of rods in early stages of growth. Here we investigated the reasons for this phenomenon by first studying the impact of auxotrophic mutations on cell shape in strains that are commonly used as genetic backgrounds for selection during strain engineering (namely: H26, H53, H77, H98, and H729) and secondly, by studying the effect o

SUBMITTER: Patro M 

PROVIDER: S-EPMC10570808 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature