Hemes on a string: insights on the functional mechanisms of PgcA from Geobacter sulfurreducens.

ABSTRACT: Microbial extracellular reduction of insoluble compounds requires soluble electron shuttles that diffuse in the environment, freely diffusing cytochromes, or direct contact with cellular conductive appendages that release or harvest electrons to assure a continuous balance between cellular requirements and environmental conditions. In this work, we produced and characterized the three cytochrome domains of PgcA, an extracellular triheme cytochrome that contributes to Fe(III) and Mn(IV) oxides reduction in Geobacter sulfurreducens. The three monoheme domains are structurally homologous, but their heme groups show variable axial coordination and reduction potential values. Electron transfer experiments monitored by NMR and visible spectroscopy show the variable extent to which the domains pr

SUBMITTER: Fernandes TM 

PROVIDER: S-EPMC10570954 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature