Simulating structurally variable nuclear pore complexes for microscopy.

ABSTRACT:

Motivation

The nuclear pore complex (NPC) is the only passageway for macromolecules between nucleus and cytoplasm, and an important reference standard in microscopy: it is massive and stereotypically arranged. The average architecture of NPC proteins has been resolved with pseudoatomic precision, however observed NPC heterogeneities evidence a high degree of divergence from this average. Single-molecule localization microscopy (SMLM) images NPCs at protein-level resolution, whereupon image analysis software studies NPC variability. However, the true picture of this variability is unknown. In quantitative image analysis experiments, it is thus difficult to distinguish intrinsically high SMLM noise from variability of the underlying structure.

Results

We introduce CIR4MICS ('c

SUBMITTER: Theiss M 

PROVIDER: S-EPMC10570993 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature