Modeling an ascending infection by Ureaplasma parvum and its cell signaling and inflammatory response at the feto-maternal interface.

ABSTRACT:

Problem

Ascending bacterial infection is associated with ∼ 40% of spontaneous preterm birth (PTB), and Ureaplasma spp. is one of the most common bacteria isolated from the amniotic fluid. Developing novel in vitro models that mimic in vivo uterine physiology is essential to study microbial pathogenesis. We utilized the feto-maternal interface organ-on-chip (FMi-OOC) device and determined the propagation of Ureaplasma parvum, and its impact on cell signaling and inflammation.

Method of study

FMi-OOC is a microphysiologic device mimicking fetal membrane/decidua interconnected through microchannels. The impact of resident decidual CD45⁺ leukocytes was also determined by incorporating them into the decidual chamber in different combinations with U. parvum. We tested t

SUBMITTER: Bento GFC 

PROVIDER: S-EPMC10571092 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature