Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis.

ABSTRACT:

Background

Zymomonas mobilis is an important industrial bacterium ideal for biorefinery and synthetic biology studies. High-throughput CRISPR-based genome editing technologies have been developed to enable targeted engineering of genes and hence metabolic pathways in the model ZM4 strain, expediting the exploitation of this biofuel-producing strain as a cell factory for sustainable chemicals, proteins and biofuels production. As these technologies mainly take plasmid-based strategies, their applications would be impeded due to the fact that curing of the extremely stable plasmids is laborious and inefficient. Whilst counterselection markers have been proven to be efficient for plasmid curing, hitherto only very few counterselection markers have been available for Z. mobilis.

Res

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC10571335 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature