Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo.

ABSTRACT:

Background

In addition to the well-known coronavirus genomes and subgenomic mRNAs, the existence of other coronavirus RNA species, which are collectively referred to as noncanonical transcripts, has been suggested; however, their biological characteristics have not yet been experimentally validated in vitro and in vivo.

Methods

To comprehensively determine the amounts, species and structures of noncanonical transcripts for bovine coronavirus in HRT-18 cells and mouse hepatitis virus A59, a mouse coronavirus, in mouse L cells and mice, nanopore direct RNA sequencing was employed. To experimentally validate the synthesis of noncanonical transcripts under regular infection, Northern blotting was performed. Both Northern blotting and nanopore direct RNA sequencing were also appl

SUBMITTER: Lin CH 

PROVIDER: S-EPMC10571414 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature