High-Content and High-Throughput Clonogenic Survival Assay Using Fluorescence Barcoding.

ABSTRACT: The Clonogenic Survival Assay (CSA) is a fundamental tool employed to assess cell survival and proliferative potential in cancer research. Despite its importance, CSA faces limitations, primarily its time- and labor-intensive nature and its binary output. To overcome these limitations and enhance CSA's utility, several approaches have been developed, focusing on increasing the throughput. However, achieving both high-content and high-throughput analyses simultaneously has remained a challenge. In this paper, we introduce LeGO-CSA, an extension of the classical CSA that employs the imaging of cell nuclei barcoded with fluorescent lentiviral gene ontology markers, enabling both high-content and high-throughput analysis. To validate our approach, we contrasted it with results from a classical

SUBMITTER: Qian H 

PROVIDER: S-EPMC10571559 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature