RNA-Seq-Based Molecular Classification Analyses in Colorectal Cancer and Synchronous Adenoma.

ABSTRACT: Colorectal cancers (CRC) are classified into consensus molecular subtypes (CMS) based on gene expression profiles. The revised classification system iCMS was proposed by considering intrinsic epithelial status, microsatellite instability (MSI), and fibrosis. This study aimed to provide molecular evidence for the adenoma-carcinoma sequence concept by examining CRC and synchronous adenomas using iCMS. Epithelial CMS cell proportion was estimated using CiberSortx, an in silico cell fractionation method that included CMS cell types among the reference cell types. A random forest (RF) model estimated the posterior probabilities of CMS classes, which were compared with the CiberSortx results. Gene expression profiles of the published iCMS signature panel were retrieved from our dataset and subje

SUBMITTER: Choi JW 

PROVIDER: S-EPMC10571664 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature