Engineered heart tissue maturation inhibits cardiomyocyte proliferative response to cryoinjury.

ABSTRACT: The cellular and molecular mechanisms that are responsible for the poor regenerative capacity of the adult heart after myocardial infarction (MI) are still unclear and their understanding is crucial to develop novel regenerative therapies. Considering the lack of reliable in vitro tissue-like models to evaluate the molecular mechanisms of cardiac regeneration, we used cryoinjury on rat Engineered Heart Tissues (rEHTs) as a new model which recapitulates in part the in vivo response after myocardial injury of neonatal and adult heart. When we subjected to cryoinjury immature and mature rEHTs, we observed a significant increase in cardiomyocyte (CM) DNA synthesis when compared to the controls. As expected, the number of mitotic CMs significantly increases in immature rEHTs when compared to ma

SUBMITTER: Ciucci G 

PROVIDER: S-EPMC10571691 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature