Expression and Activity of the NF-κB Subunits in Chronic Lymphocytic Leukaemia: A Role for RelB and Non-Canonical Signalling.

ABSTRACT:

Background

Canonical NF-κB signalling by p65 (RelA) confers chemo-resistance and poor survival in chronic lymphocytic leukaemia (CLL). The role of non-canonical NF-κB signalling (leading to RelB and p52 subunit activation) in CLL is less understood, but given its importance in other B-cell tumour types, we theorised that RelB and p52 may also contribute to the pathology of CLL.

Methods

DNA binding activity of all five NF-kB subunits, p65, p50, RelB, p52, and c-Rel, was quantified using ELISA and correlated to ex vivo chemoresistance, CD40L-stimulated signalling (to mimic the lymph node microenvironment), and clinical data.

Results

Importantly, we show for the first time that high basal levels of RelB DNA binding correlate with nuclear RelB protein expression and are a

SUBMITTER: Mulligan EA 

PROVIDER: S-EPMC10571822 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature