A Novel Role of Connective Tissue Growth Factor in the Regulation of the Epithelial Phenotype.

ABSTRACT:

Background

Epithelial-mesenchymal transition (EMT) is a biological process where epithelial cells lose their adhesive properties and gain invasive, metastatic, and mesenchymal properties. Maintaining the balance between the epithelial and mesenchymal stage is essential for tissue homeostasis. Many of the genes promoting mesenchymal transformation have been identified; however, our understanding of the genes responsible for maintaining the epithelial phenotype is limited. Our objective was to identify the genes responsible for maintaining the epithelial phenotype and inhibiting EMT.

Methods

RNA seq was performed using an vitro model of EMT. CTGF expression was determined via qPCR and Western blot analysis. The knockout of CTGF was completed using the CTGF sgRNA CRISPR/CAS9. T

SUBMITTER: Gogoi RP 

PROVIDER: S-EPMC10571845 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature