Characterization of the Intraclonal Complexity of Chronic Lymphocytic Leukemia B Cells: Potential Influences of B-Cell Receptor Crosstalk with Other Stimuli.

ABSTRACT: Chronic lymphocytic leukemia (CLL) clones contain subpopulations differing in time since the last cell division ("age"): recently born, proliferative (PF; CXCR4^DimCD5^Bright), intermediate (IF; CXCR4^IntCD5^Int), and resting (RF; CXCR4^BrightCD5^Dim) fractions. Herein, we used deuterium (²H) incorporation into newly synthesized DNA in patients to refine the kinetics of CLL subpopulations by characterizing two additional CXCR4/CD5 fractions, i.e., double dim (DDF; CXCR4^DimCD5^Dim) and double bright (DBF; CXCR4^BrightCD5^Bright); and intraclonal fractions differing in surface membrane (sm) IgM and IgD densities. Although DDF was enriched in recently divided cells and DBF in older

SUBMITTER: Mazzarello AN 

PROVIDER: S-EPMC10571896 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature