Purine Biosynthesis Pathways Are Required for Myogenesis in Xenopus laevis.

ABSTRACT: Purines are required for fundamental biological processes and alterations in their metabolism lead to severe genetic diseases associated with developmental defects whose etiology remains unclear. Here, we studied the developmental requirements for purine metabolism using the amphibian Xenopus laevis as a vertebrate model. We provide the first functional characterization of purine pathway genes and show that these genes are mainly expressed in nervous and muscular embryonic tissues. Morphants were generated to decipher the functions of these genes, with a focus on the adenylosuccinate lyase (ADSL), which is an enzyme required for both salvage and de novo purine pathways. adsl.L knockdown led to a severe reduction in the expression of the myogenic regulatory factors (MRF

SUBMITTER: Duperray M 

PROVIDER: S-EPMC10571971 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature