Novel In Vitro Models for Cell Differentiation and Drug Transport Studies of the Human Intestine.

ABSTRACT: The most common in vitro model for absorption, distribution, metabolism, and excretion (ADME) purposes is currently the Caco-2 cell line. However, clear differences in gene and protein expression towards the small intestine and an, at best, fair prediction accuracy of intestinal drug absorption restrict the usefulness of a model for intestinal epithelial cells. To overcome these limitations, we evaluated a panel of low-passaged patient-derived colorectal cancer cell lines of the HROC collection concerning similarities to small intestinal epithelial cells and their potential to predict intestinal drug absorption. After initial screening of a larger panel, ten cell lines with confluent outgrowth and long-lasting barrier-forming potential were further characterized in close detail. Tight junc

SUBMITTER: Przybylla R 

PROVIDER: S-EPMC10572004 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature