K⁺-Dependent Photocycle and Photocurrent Reveal the Uptake of K⁺ in Light-Driven Sodium Pump.

ABSTRACT: Engineering light-controlled K⁺ pumps from Na⁺-pumping rhodopsins (NaR) greatly expands the scope of optogenetic applications. However, the limited knowledge regarding the kinetic and selective mechanism of K⁺ uptake has significantly impeded the modification and design of light-controlled K⁺ pumps, as well as their practical applications in various fields, including neuroscience. In this study, we presented K⁺-dependent photocycle kinetics and photocurrent of a light-driven Na⁺ pump called Nonlabens dokdonensis rhodopsin 2 (NdR2). As the concentration of K⁺ increased, we observed the accelerated decay of M intermediate in the wild type (WT) through flash photolysis. In 100 mM KCl, the lifetime of the M dec

SUBMITTER: Xu J 

PROVIDER: S-EPMC10572131 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature