Mapping Cell-in-Cell Structures in Oral Squamous Cell Carcinoma.

ABSTRACT: Cell-in-cell (CIC) structures contribute to tumor aggressiveness and poor prognosis in oral squamous cell carcinoma (OSCC). In vitro 3D models may contribute to the understanding of the underlying molecular mechanisms of these events. We employed a spheroid model to study the CIC structures in OSCC. Spheroids were obtained from OSCC (HSC3) and cancer-associated fibroblast (CAF) lines using the Nanoshuttle-PL^TM bioprinting system (Greiner Bio-One). Spheroid form, size, and reproducibility were evaluated over time (Evos^TM XL; ImageJ version 1.8). Slides were assembled, stained (hematoxylin and eosin), and scanned (Axio Imager Z2/VSLIDE) using the OlyVIA System (Olympus Life Science) and ImageJ software (NIH) for cellular morphology and tumor zone formation (hypoxia and/

SUBMITTER: Siquara da Rocha LO 

PROVIDER: S-EPMC10572403 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature