The Pro-Oncogenic Protein IF₁ Promotes Proliferation of Anoxic Cancer Cells during Re-Oxygenation.

ABSTRACT: Cancer cells overexpress IF₁, the endogenous protein that inhibits the hydrolytic activity of ATP synthase when mitochondrial membrane potential (Δμ_H⁺) falls, as in ischemia. Other roles have been ascribed to IF₁, but the associated molecular mechanisms are still under debate. We investigated the ability of IF₁ to promote survival and proliferation in osteosarcoma and colon carcinoma cells exposed to conditions mimicking ischemia and reperfusion, as occurs in vivo, particularly in solid tumors. IF₁-silenced and parental cells were exposed to the FCCP uncoupler to collapse Δμ_H⁺ and the bioenergetics of cell models were validated. All the uncoupled cells preserved mitochondrial mass, but the implemented m

SUBMITTER: Righetti R 

PROVIDER: S-EPMC10572598 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature