Molecular Mechanism of STIL Coiled-Coil Domain Oligomerization.

ABSTRACT: Coiled-coil domains (CCDs) play key roles in regulating both healthy cellular processes and the pathogenesis of various diseases by controlling protein self-association and protein-protein interactions. Here, we probe the mechanism of oligomerization of a peptide representing the CCD of the STIL protein, a tetrameric multi-domain protein that is over-expressed in several cancers and associated with metastatic spread. STIL tetramerization is mediated both by an intrinsically disordered domain (STIL_400-700) and a structured CCD (STIL CCD_718-749). Disrupting STIL oligomerization via the CCD inhibits its activity in vivo. We describe a comprehensive biophysical and structural characterization of the concentration-dependent oligomerization of STIL CCD peptide. We co

SUBMITTER: Shamir M 

PROVIDER: S-EPMC10572602 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature