Qualitative and Quantitative Detection of CRISPR-Associated Cas Gene in Gene-Edited Foods.

ABSTRACT: Effective regulation of gene-edited products and resolution of public concerns are the prerequisites for the industrialization of gene-edited crops and their derived foods. CRISPR-associated protein, the core element of the CRISPR system, requires to be regulated. Thus, there is an urgent need to establish qualitative and quantitative detection methods for the Cas gene. In the present study, the primers and probes were designed and screened for Cas12a (Cpf1), which is the most commonly used target site in gene editing; we performed PCR system optimization, determined the optimal primer concentration and annealing temperature, and established qualitative PCR and quantitative PCR (qPCR) assays for detecting Cpf1 in gene editing by specificity and sensitivity tests

SUBMITTER: Ding L 

PROVIDER: S-EPMC10572612 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature