Factors Influencing Marker Expressions of Cultured Human Cord Blood-Derived Mast Cells.

ABSTRACT: Mast cells (MCs) are tissue-resident immune cells of a hematopoietic origin that play vital roles in innate and adaptive immunity. Human MCs can be isolated and differentiated from various tissue sources, including cord blood, when supplemented with cytokines such as stem cell factor, interleukin 3, and interleukin 6. Our current research study has shown significant differences in the marker expressions of human cord blood-derived mast cells (hCBMCs) based on donor dependency and the type of medium used for culturing and differentiation. These findings are particularly relevant given the challenges of obtaining specialty media influencing MC phenotypic marker expressions. We found that hCBMCs cultured in StemSpanTM-XF medium had a moderate expression of mast/stem cell growth factor recepto

SUBMITTER: Alimohammadi S 

PROVIDER: S-EPMC10573221 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature