Persisting roadblocks in arthropod monitoring using non-destructive metabarcoding from collection media of passive traps.

ABSTRACT:

Background

Broad-scale monitoring of arthropods is often carried out with passive traps (e.g., Malaise traps) that can collect thousands of specimens per sample. The identification of individual specimens requires time and taxonomic expertise, limiting the geographical and temporal scale of research and monitoring studies. DNA metabarcoding of bulk-sample homogenates has been found to be faster, efficient and reliable, but the destruction of samples prevents a posteriori validation of species occurrences and relative abundances. Non-destructive metabarcoding of DNA extracted from collection medium has been applied in a limited number of studies, but further tests of efficiency are required with different trap types and collection media to assess the consistency of the

SUBMITTER: Sire L 

PROVIDER: S-EPMC10573316 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature