N⁶-Methyladenine Progressively Accumulates in Mitochondrial DNA during Aging.

ABSTRACT: N⁶-methyladenine (6mA) in the DNA is a conserved epigenetic mark with various cellular, physiological and developmental functions. Although the presence of 6mA was discovered a few years ago in the nuclear genome of distantly related animal taxa and just recently in mammalian mitochondrial DNA (mtDNA), accumulating evidence at present seriously questions the presence of N⁶-adenine methylation in these genetic systems, attributing it to methodological errors. In this paper, we present a reliable, PCR-based method to determine accurately the relative 6mA levels in the mtDNA of Caenorhabditis elegans, Drosophila melanogaster and dogs, and show that these levels gradually increase with age. Furthermore, daf-2(-)-mutant worms, which are d

SUBMITTER: Sturm A 

PROVIDER: S-EPMC10573865 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature