Ex Vivo Colonic Fermentation of NUTRIOSE^® Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects.

ABSTRACT: NUTRIOSE^® (Roquette, Lestrem, France) is a resistant dextrin with well-established prebiotic effects. This study evaluated the indirect effects of pre-digested NUTRIOSE^® on host immune response and gut barrier integrity. Fecal samples from eight healthy donors were inoculated in a Colon-on-a-plate^® system (ProDigest, Ghent, Belgium) with or without NUTRIOSE^® supplementation. Following 48 h fermentation, colonic suspensions were tested in a Caco-2/THP1-Blue™ co-culture system to determine their effects on gut barrier activity (transepithelial electrical resistance) and immune response following lipopolysaccharide stimulation. Additionally, changes in short-chain fatty acid levels (SCFA) and microbial community composition following a 48 h fermenta

SUBMITTER: Perreau C 

PROVIDER: S-EPMC10574048 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature