Tailoring Effective Phage Cocktails for Long-Term Lysis of Escherichia coli Based on Physiological Properties of Constituent Phages.

ABSTRACT:

Background

Bacteriophage (phage) therapy has regained attention as an alternative to antimicrobial agents for eliminating bacteria; however, the emergence of phage-resistant bacteria during the therapy is a major concern. One method to control this emergence is to create a cocktail composed of multiple phages.

Materials and methods

In this study, we isolated 28 phages infecting Escherichia coli and evaluated their bacteriolysis (lysis) activity, lytic spectrum, adsorption rate constant, burst size, and titer of a 1-day incubation, followed by clustering of the phages based on these physiological characteristics.

Results

The variation in lysis onset time and duration was more significant for cocktails of phages from different clusters than for phage cocktails fr

SUBMITTER: Kaneko T 

PROVIDER: S-EPMC10574527 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature