Cell-Penetrating Milk-Derived Peptides with a Non-Inflammatory Profile.

ABSTRACT: Milk-derived peptides are known to confer anti-inflammatory effects. We hypothesised that milk-derived cell-penetrating peptides might modulate inflammation in useful ways. Using computational techniques, we identified and synthesised peptides from the milk protein Alpha-S1-casein that were predicted to be cell-penetrating using a machine learning predictor. We modified the interpretation of the prediction results to consider the effects of histidine. Peptides were then selected for testing to determine their cell penetrability and anti-inflammatory effects using HeLa cells and J774.2 mouse macrophage cell lines. The selected peptides all showed cell penetrating behaviour, as judged using confocal microscopy of fluorescently labelled peptides. None of the peptides had an effect on either t

SUBMITTER: Agoni C 

PROVIDER: S-EPMC10574647 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature