Fingerprinting of Proteases, Protease Inhibitors and Indigenous Peptides in Human Milk.

ABSTRACT: The presence of proteases and their resulting level of activity on human milk (HM) proteins may aid in the generation of indigenous peptides as part of a pre-digestion process, of which some have potential bioactivity for the infant. The present study investigated the relative abundance of indigenous peptides and their cleavage products in relation to the abundance of observed proteases and protease inhibitors. The proteomes and peptidomes in twelve HM samples, representing six donors at lactation months 1 and 3, were profiled. In the proteome, 39 proteases and 29 protease inhibitors were identified in 2/3 of the samples. Cathepsin D was found to be present in higher abundance in the proteome compared with plasmin, while peptides originating from plasmin cleavage were more abundant than pe

SUBMITTER: Thesbjerg MN 

PROVIDER: S-EPMC10574734 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature