Optimized nucleus isolation protocol from frozen mouse tissues for single nucleus RNA sequencing application.

ABSTRACT: The single cell RNA sequencing technique has been particularly used during the last years, allowing major discoveries. However, the widespread application of this analysis has showed limitations. Indeed, the direct study of fresh tissues is not always feasible, notably in the case of genetically engineered mouse embryo or sensitive tissues whose integrity is affected by classical digestion methods. To overcome these limitations, single nucleus RNA sequencing offers the possibility to work with frozen samples. Thus, single nucleus RNA sequencing can be performed after genotyping-based selection on samples stocked in tissue bank and is applicable to retrospective studies. Therefore, this technique opens the field to a wide range of applications requiring adapted protocols for nucleus isolati

SUBMITTER: Minati MA 

PROVIDER: S-EPMC10575574 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature