High-resolution analysis of bound Ca²⁺ in neurons and synapses.

ABSTRACT: Calcium (Ca²⁺) is a well-known second messenger in all cells, and is especially relevant for neuronal activity. Neuronal Ca²⁺ is found in different forms, with a minority being freely soluble in the cell and more than 99% being bound to proteins. Free Ca²⁺ has received much attention over the last few decades, but protein-bound Ca²⁺ has been difficult to analyze. Here, we introduce correlative fluorescence and nanoscale secondary ion mass spectrometry imaging as a tool to describe bound Ca²⁺ As expected, bound Ca²⁺ is ubiquitous. It does not correlate to free Ca²⁺ dynamics at the whole-neuron level, but does correlate significantly to the intensity of markers for GABAergic pre-synapse and glutamatergic post-synaps

SUBMITTER: Bonnin EA 

PROVIDER: S-EPMC10575792 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature