Deciphering variations in the endocytic uptake of a cell-penetrating peptide: the crucial role of cell culture protocols.

ABSTRACT: Delivery tools, including cell-penetrating peptides (CPPs), are often inefficient due to a combination of poor endocytosis and endosomal escape. Aspects that impact the delivery of CPPs are typically characterized using tissue culture models. One problem of using cell culture is that cell culture protocols have the potential to contribute to endosomal uptake and endosomal release of CPPs. Hence, a systematic study to identify which aspects of cell culturing techniques impact the endocytic uptake of a typical CPP, the TMR-TAT peptide (peptide sequence derived from HIV1-TAT with the N-terminus labeled with tetramethylrhodamine), was conducted. Aspects of cell culturing protocols previously found to generally modulate endocytosis, such as cell density, washing steps, and cell aging, did not a

SUBMITTER: Diaz J 

PROVIDER: S-EPMC10575844 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature