Structure of the transcription open complex of distinct σ^I factors.

ABSTRACT: Bacterial σ^I factors of the σ⁷⁰-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ^I paralogues in some cellulolytic bacteria have been shown to be responsible for the regulation of the cellulosome, a multienzyme complex that mediates efficient cellulose degradation. Here, we report two structures at 3.0 Å and 3.3 Å of two transcription open complexes formed by two σ^I factors, SigI1 and SigI6, respectively, from the thermophilic, cellulolytic bacterium, Clostridium thermocellum. These structures reveal a unique, hitherto-unknown recognition mode of bacterial transcriptional promoters, both with respect to domain organization and bind

SUBMITTER: Li J 

PROVIDER: S-EPMC10575876 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature