Characterization and regulation of salt upregulated cyclophilin from a halotolerant strain of Penicillium oxalicum.

ABSTRACT: Penicillium species are an industrially important group of fungi. Cyclophilins are ubiquitous proteins and several members of this family exhibit peptidyl-prolyl cis-trans isomerase (PPIase) activity. We had earlier demonstrated that the salt-induced PPIase activity in a halotolerant strain of P. oxalicum was associated with enhanced expression of a cyclophilin gene, PoxCYP18. Cloning and characterization of PoxCYP18 revealed that its cDNA consists of 522 bp encoding a protein of 173 amino acid residues, with predicted molecular mass and pI values of 18.91 kDa and 8.87, respectively. The recombinant PoxCYP18 can catalyze cis-trans isomerization of peptidyl-prolyl bond with a catalytic efficiency of 1.46 × 10⁷ M^-1 s^-1 and is inhibited specifically only by cy

SUBMITTER: Singh M 

PROVIDER: S-EPMC10575979 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature