Trace impurities in sodium phosphate influences the physiological activity of Escherichia coli in M9 minimal medium.

ABSTRACT: In the field of applied microbiology, reproducibility and experimental variability are important factors that influence both basic research as well as process development for industrial applications. Experimental reproducibility and accuracy depend not only on culture conditions such as temperature and aeration but also on raw materials and procedures used for media preparation. The M9 minimal medium is one of the most common synthetic media for culturing Escherichia coli and other bacteria. This synthetic medium can be used to observe and evaluate the physiological activity of microbes under minimal nutritional requirements and determine the limiting factor for the desired phenotype. Although one of the advantages using the M9 medium is that its composition can be modulated, it is difficu

SUBMITTER: Soma Y 

PROVIDER: S-EPMC10576033 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature