Physiological and stoichiometric characterization of ethanol-based chain elongation in the absence of short-chain carboxylic acids.

ABSTRACT: Hexanoate is a valuable chemical that can be produced by microorganisms that convert short-chain- to medium-chain carboxylic acids through a process called chain elongation. These microorganisms usually produce mixtures of butyrate and hexanoate from ethanol and acetate, but direct conversion of ethanol to hexanoate is theoretically possible. Steering microbial communities to ethanol-only elongation to hexanoate circumvents the need for acetate addition and simplifies product separation. The biological feasibility of ethanol elongation to hexanoate was validated in batch bioreactor experiments with a Clostridium kluyveri-dominated enrichment culture incubated with ethanol, acetate and butyrate in different ratios. Frequent liquid sampling combined with high-resolution off-gas measurements

SUBMITTER: Allaart MT 

PROVIDER: S-EPMC10576071 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature