Multilayer three-dimensional filter paper constructs for the culture and analysis of aortic valvular interstitial cells.

ABSTRACT: Culturing aortic valvular interstitial cells in an environment that models the aortic valve is an essential step towards understanding the progression of calcific aortic valve disease. Here the adaption of a three-dimensional (3-D) stacked paper-based culture system is presented for analyzing valve cells in a thick collagen gel matrix. Filter paper layers, modeled after a 96-well plate design, were printed with a wax well-plate template and then seeded with valve cell and collagen mixtures that quickly gelled into 3-D cultures. Stacking these layers permitted extensive customization of culture thickness and cell density profiles to model the full thickness of native valve tissue. Aortic valvular interstitial cells seeded into the paper-based constructs consistently demonstrated high surviv

SUBMITTER: Sapp MC 

PROVIDER: S-EPMC10576252 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature